Figure 6
From: NADPH oxidase 4 deficiency increases tubular cell death during acute ischemic reperfusion injury
Protein glutathionylation and glutathione amount are decreased under NOX4 deficiency.
(A,B) Western blot analysis of protein glutathionylation performed on kidney cortex from WT and NOX4 mice in non-reduced conditions. Representative image (A) and glutathionylated proteins densitometric quantification (B) are shown. Results are expressed as the mean of densitometric values over the mean of densitometric value obtained in WT ± SEM, ns p > 0.05, *p < 0.05 (WT, n = 6, NOX4 KO, n = 5). (C,D) Western blot analysis of protein glutathionylation performed on MEF cells from WT and NOX4 KO mice under non-reducing conditions. Representative image (C) and glutathionylated proteins densitometric quantification (D) are shown. Results are expressed as the mean of densitometric values over the mean of densitometric value obtained in WT cells ± SEM, (n = 3), ns p > 0.05, *p < 0.05, **p < 0.005. (E) Glutathione (GSH) amount measurement performed on mCCDcl1 cells transfected or not with a siRNA targeting NOX4. Results are expressed as ratio of relative quantity over the mean value obtained in WT or scramble RNA transfected mCCDcl1 cells ± SEM, (n = 3), ns p > 0.05, *p < 0.05.
