Figure 7
From: NADPH oxidase 4 deficiency increases tubular cell death during acute ischemic reperfusion injury
Mitochondrial stability is modified by NOX4 depletion.
MEF and mCCDcl1 mitochondrial membrane potential was assessed by JC-1 assay based on a dye exhibiting a potential dependent accumulation in mitochondria indicated by a fluorescence emission shift from red to green. Fluorescence shift is more important in KO or silenced NOX4 cells than Wt cells. Representative quantification (A,B) of JC-1 in WT and MEF KOs are shown. Results are expressed as red/green ratio ± SEM, (n = 3), ns p > 0.05, *p < 0.05. Representative quantification (C,D) of JC-1 in Sbl and siNOX4 mCCDcl1 are shown. Results are expressed as red/green ratio ± SEM, (n = 3), ns p > 0.05, *p < 0.05. (E,F) Real time PCR analysis of NQO1 (B) and Thioredoxin (TXN) (C) performed on MEF cells derived from WT and NOX4 KO mice. Results are expressed as relative expression compared to WT cells ± S EM, (n = 3), ns p > 0.05, *p < 0.05, **p < 0.005.
