Figure 2 | Scientific Reports

Figure 2

From: Genomic duplication and translocation of reactivation transactivator and bZIP-homolog genes is a conserved event in alcelaphine herpesvirus 1

Figure 2

Localization of the duplicated region in the BAC clone by Southern blotting.

(A) Southern blot of the BAC clone digested with various restriction endonucleases and hybridized with ORF50 or TR probes. The sizes (anticipated and deduced) of bands from LUR-C500DT or TR-C500DT are shown in black and red, respectively. The ORF50 probe was produced by PCR using primers C500-1 and C500-224. The TR probe was produced after AvrII/SmaI digestion of plasmid pBluescribe-C500RE BS15 and purification of the band corresponding to a 235 bp sequence of TR (nt 490–724 of TR). EtBr indicates ethidium bromide-stained lanes prior to blotting. Entire gels and blots are shown with ladders of medium (MW) and high molecular weight (High MW) standards. (B) Diagram of the BAC clone showing the positions of restriction sites used to locate the duplicated region. Locations of the ORF50 and TR probes used in A are shown by green and blue lines, respectively.

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