Figure 5

Effects of the duplicated region on viral fitness in vitro.
(A) Multistep growth curves of WT, LUR-C500DT− and TR-C500DT− viruses in BT cells. Data are means ± S.D. from triplicate independent measurements and representative of two independent experiments with similar results. Statistical analysis was done by two-way ANOVA with the Bonferroni test for multiple comparisons; *p ≤ 0.01, **p ≤ 0.05, ***p ≤ 0.001. (B) ORF50 mRNA expression in MDBK cells 3 days post-infection (moi = 0.01) with WT, LUR-C500DT− or TR-C500DT− viruses. Expression levels are given as absolute copy numbers from an RT reaction of 1 μg of cytoplasmic RNA and as normalized ORF50/ORF25 ratios. Data are means ± S.D. from measurements carried out in triplicate. Statistical analysis was done by Student t test, ***p ≤ 0.001.