Table 2 Oligonucleotides used in this study.

From: Genomic duplication and translocation of reactivation transactivator and bZIP-homolog genes is a conserved event in alcelaphine herpesvirus 1

Sequence

Primer

Primer sequence (5′-3′)

β-lactamase gene

LUR-C500DT-AmpR-Fwd

GTCCTTTTCAATCATATTGTCTTCTGTTTGAATCAGGCTAACTAGCACCAGTGCGCGGAACCCCTATTTG

LUR-C500DT-AmpR-Rev

GCTCTGGTGGGGAAAGGTTAGGTTGCCAATTGTTATATAAACCAATTGCTTTACCAATGCTTAATCAGTG

galK sequence

TR-C500DT-galK-Fwd

AAGAGGCCAGCACAGTAGCAGTCATCTGTTTTACAAAAAGTCCCCCTTAACCTGTTGACAATTAATCATCGGCA

TR-C500DT-galK-Rev

TATAGAAAGCCAAGCAGCCCAGGGCAAAGGCAGCTATCATTAAATTTGTGTCAGCACTGTCCTGCTCCTT

TR-C500DT

C500DT-clean-Fwd

AGAGGCCAGCACAGTAGCAGTCATCTGTTTTACAAAAAGTCCCCCTTAACACAAATTTAATGATAGCTGCCTTTGCCCTGGGCTGCTTGGCTTTCTAT

C500DT-clean-Rev

ATAGAAAGCCAAGCAGCCCAGGGCAAAGGCAGCTATCATTAAATTTGTGTTAAGGGGGACTTTTTGTAAAACAGATGACTGCTACTGTGCTGGCCTCT

galK sequence

LUR-C500DT-galK-Fwd

GTCCTTTTCAATCATATTGTCTTCTGTTTGAATCAGGCTAACTAGCACCACCTGTTGACAATTAATCATCGGCA

LUR-C500DT-galK-Rev

GCTCTGGTGGGGAAAGGTTAGGTTGCCAATTGTTATATAAACCAATTGCTTCAGCACTGTCCTGCTCCTT

LUR-C500DT

LUR-C500DT-Fwd

GTCCTTTTCAATCATATTGTC

LUR-C500DT-Rev

GCTCTGGTGGGGAAAGGTTAG

C500DT/left TR-LUR junction

A6-ATG-Fwd

ATGCATAAGCACTCTGCTACAGC

Left-end-C500-Rev

GGCTATCTGCTGCGCGGCGC

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