Figure 2
From: Identification of a CD133−CD55− population functions as a fetal common skeletal progenitor
Only CD105+CD90.1−CD133−CD55− fetal progenitors generated both osteoblast and chondrocyte in vitro.
Fetal CD105+CD90.1− osteochondral progenitors were further sorted based on CD133 and CD55 expression and cultured for one month. (A–C) Bright-field image of cultured CD133−CD55− (A), CD133+CD55− (B) and CD133+CD55+ (C) progenitors. Only CD133−CD55− cell formed both the chondrocytes cluster and osteoblast. (D) Upper panels: The cultured CD133−CD55− cells were stained for the expression of Col2 (red) and osteocalcin (green). Lower panels: A representative chondrocyte cluster (red). (E) Summary of single cell colony forming assay of the three fetal skeletal progenitors. (F–H) Representative images of the colonies derived from cultured single cell of CD133−CD55− (F), CD133+CD55− (G) and CD133+CD55+ (H) progenitors. (I) Representative FACS profiles of CD133−CD55− cells 2, 4, 6 and 7 days in culture.
