Figure 6
From: Identification of a CD133−CD55− population functions as a fetal common skeletal progenitor
CD133−CD55− common progenitors differentiated into Sca1+ mesenchyme cells in ectopic BM niche.
(A) Representative iCys scanned section of the KC graft derived from GFP labeled CD133−CD55− progenitor was stained with antibody against Sca1. Cells expressing both GFP and Sca1 were indicated by triangles, stars and arrows. The triangles label cells located in endosteum. The stars label the cells in perivascular regions. The arrows label the cells in stromal regions. (B) The expression of Sca1 in CD133−CD55− common progenitors determined by FACS analysis. (C) Representative brightfield (upper) and H.E section of the KC graft of sorted CD133−CD55−Sca1− cells one month after transplantation. Arrows indicate ectopic bones; star indicates the bone marrow. (D) GFP image of KC graft derived from GFP labeled CD133−CD55−Sca1− cells. (E) Representative FACS analysis of Sca−1 expression in GFP+ graft cells. (F) Section of GFP+ KC graft stained with Sca1. Arrows indicate the cells expressing both GFP and Sca−1. Blue dot line outlines the KC graft.
