Table 3 Mineral density as calculated from the Micro-CT scans.

From: Stress physiology and weapon integrity of intertidal mantis shrimp under future ocean conditions

Segment

Treatment

T.A. (mm2) or T.V. (mm3)*

Obj.A. (mm2) or Obj.V. (mm3)*

Obj.A./T.A. or Obj.V./T.V.* (%)

Obj.Th. (mm) or Obj.S. (mm2)*

T. M. D. (gHA/cm3)

merus

ambient

11.734

0.368

3.13

0.063

0.631

reduced pH

11.734

0.489

4.17

0.076

0.694

reduced pH/increased temp

11.734

0.387

3.30

0.062

0.663

carapace

ambient

5.056

0.191

3.78

0.061

0.562

reduced pH

5.056

0.186

3.67

0.063

0.634

reduced pH/increased temp

5.056

0.155

3.06

0.058

0.613

dactyl*

low density

ambient

2.997

2.089

69.70

14.359

1.294

reduced pH

3.063

2.019

65.91

14.132

1.344

reduced pH/increased temp

2.844

1.490

52.38

17.929

1.455

high density

ambient

2.997

0.747

24.91

12.488

1.877

reduced pH

3.063

0.879

28.70

13.978

1.879

reduced pH/increased temp

2.844

1.011

35.54

25.348

1.853

  1. The carapace and merus were quantified via a 2D analysis by integrating 2D slices in the region of interest as defined by the total area (T.A.), while the dactyl was 3D analyzed in the total volume (T.V.) of interest. Area metrics presented refer to the merus and carapace. Volume metrics refer to the dactyl as indicated by the*. Obj.A or Obj.V. is the total object area or volume obtained inside the T.A. or T.V., respectively. Obj.A./T.A. is the object area divided by the total area, and Obj.V./T.V. is the object volume divided by the total volume; both metrics have the same units. Obj.Th. is the thickness of the object and Obj.S. is the surface area of the object’s volume. T.M.D. is the true mineral density calculated by correlating attenuation coefficients from images of sample and hydroxyapatite (HA) rods of known densities. The units for T.M.D are the same for the merus, carapace, and dactyl.
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