Figure 1

Biophysical and PK properties of MEDI-578 and MEDI1912.

(a) Size exclusion chromatography-HPLC (SEC-HPLC) elution profiles of MEDI-578 (red) and MEDI1912 (blue), each 1 mg/mL, at 280 nm (mAu) (0.1 M sodium phosphate, 0.1 M sodium sulphate, pH 6.8). Grey lines show calibrants used: Thyroglobulin (1), IgG (2), Ovalbumin (3) and Vitamin B₁₂ (4). (b) Sedimentation-velocity analytical ultracentrifugation (SV-AUC) of MEDI1912 (1 mg/mL, 20 mM sodium succinate, 125 mM arginine, pH 6). (c) MEDI-578 and MEDI1912 PK profiles following intravenous administration of a 3.0 mg/kg, 0.3 mg/kg or 0.03 mg/kg dose to rats (n = 3). (d) MEDI-578 and MEDI1912 PK profiles following intravenous administration of a 1 mg/kg dose to cynomolgus monkeys (n = 3). Data points represent the mean ± SD.