Table 2 HP-SEC column interaction of MEDI1912 variants.
Antibody | Mutations | HP-SEC monomer retention time (mins) |
|---|---|---|
Reference IgG | N/A | 8.7 |
MEDI-578 | N/A | 8.7 |
MEDI1912 (WFL) | — | 15.0 |
Mutant 1 (WFT) | L56T | 12.3 |
Mutant 2 (WTL) | F31T | 10.3 |
Mutant 3 (SFL) | W30S | 10.3 |
Mutant 4 (SFT) | W30S, L56T | 9.7 |
Mutant 5 (STL) | W30S, F31T | 9.0 |
Mutant 6 (STT) | W30S, F31T, L56T | 8.9 |
