Figure 4
Silencing Nrf2 enhanced TGF-β1-induced EMT in RLE-6TN cells.
Nrf2 siRNA were transfected in cells before stimulated with TGF-β1 for 24 h, then cell lysates were collected and the relative proteins were determined by Western blot. The representative bands were obtained from different gels for repeated experiments. β-actin was used as an internal reference for relative quantification. Data were expressed as mean ± SD (n = 3–4 per group), **P < 0.01 compared with vehicle group; ##P < 0.01 compared with TGF-β1 group.
