Figure 3
From: HOPX functions as a tumour suppressor in head and neck cancer
HOPX promoter methylation in HNSCC.
Quantitative analysis of HOPX-β promoter methylation in OSCC and NPC cell lines with normal oral keratinocytes and immortalised nasopharyngeal epithelial cell lines (A). Treatment of H413 OSCC cells with the demethylating drug, zebularine, for 5–10 days induced re-expression of HOPX (B). HOPX-β promoter methylation was associated with a reduction of HOPX expression as determined by comparing expression in matched tumour and normal data within the TCGA HNSCC dataset (C). Hypermethylated cases are shown in red, those with higher expression than their matched normal are shown in green, with the remainder shown in blue. Kaplan Meier survival analysis showed there was a significant association between HOPX-β promoter methylation and worse overall survival (D).
