Figure 4
From: A genome-wide screen of bacterial mutants that enhance dauer formation in C. elegans
Feeding bacterial cyaA mutants alters DAF-7 expression in C. elegans.
(a) A scatter plot of log2 transcript probe intensities for daf-2(e1370) versus N2, and K-12 (control) versus cyaA fed control worm comparisons. Genes which show only p-values of <0.05 and fold changes >1.3 and <0.8 were shortlisted. (b) Relative expression of daf-7 mRNA in N2 animals on feeding K-12(control), cyaA mutant bacteria, and cyaA mutant bacteria supplemented with cAMP(2 mM). (c,d) cAMP induces the expression of DAF-7 in ASI neuron. The fraction of animals show DAF-7 expression at L1 and L2 stage on feeding K-12 (control), cyaA mutant bacteria, and cyaA mutant bacteria supplemented with cAMP (2 mM). The data is represented as percent mean ± S.D. of greater than 3 biological replicates, n > 200, **P < 0.0001.
