Figure 5
From: A genome-wide screen of bacterial mutants that enhance dauer formation in C. elegans
Bacterial cyaA mutants affect dauer formation through TGF-β pathway and DAF-16.
(a–e) Dauer formation of daf-2(e1370), daf-7(e1372), daf-11(m47) and daf-2(e1370); daf-3(e1376), daf-2(e1370); daf-5(e1386) animals on feeding K-12 (control), cyaA mutant bacteria, and cyaA mutant bacteria supplemented with cAMP (2 mM). (f) DAF-16::GFP worms in the N2 background on feeding K-12 (control), cyaA mutant bacteria, and cyaA mutant bacteria supplemented with cAMP(2 mM). Quantification of DAF-16::GFP localization on feeding K-12 (control), cyaA mutant bacteria, and cyaA mutant bacteria supplemented with cAMP(2 mM). In each case, the data is represented as mean percent ± S.D of three replicates, **P < 0.0001, n > 200.
