Figure 6
A schedule of ketamine administration and behavioral assessment.
Mice received ketamine daily administration for 90 or 180 days (3 or 6 months respectively). For the three months administration paradigm, Morris Water Maze tests began on Day 91 with daily training sessions that lasted until Day on 98 of the study, Radial Arm Maze tests started on Day 91 with daily training sessions that lasted until on Day 93. For the six months administration paradigm, Morris Water Maze tests began on Day 181 and lasted until Day 188 of the study, Radial Arm Maze tests started on Day 181 and lasted until on Day 183. We surveyed the first sessions of escape latency (Day 93–97) and probe trials (Day 98) of mice on three months administration paradigm and the second sessions of escape latency (Day 183–187) and probe trials (Day 188) of mice on six months administration paradigm, to assess for spatial reference memory performance. Meanwhile, trials for the total errors and time spent to accomplish a session (Day 92–93 and Day 182–183) of mice in the Radial Arm Maze test were conducted to assess for spatial working memory performance as well. Animals were euthanized on Day 94, 99, 184 and 189 of the study, and brain tissue was dissected, collected, and stored in the −80 °C freezer.
