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Figure 1

From: Acid-sensing ion channels are expressed in the ventrolateral medulla and contribute to central chemoreception

Figure 1

Expression of ASIC1a and ASIC2a in the medulla of adult and neonatal SD rat.

(a) RNA levels of ASIC1a and ASIC2a were measured by real-time qualitative PCR. RNA levels of ASIC1a and ASIC2a were significantly decreased in adult rats as compared to their neonatal rats. The RNA expression levels of ASIC2a is higher than ASIC1a. RNA level of ASIC1a of neonatal rats was set as 1.0, all others were normalized to the ASIC1a of neonatal rats. *p < 0.05, **p < 0.01, ***p < 0.001, n = 4. (b) Protein of ASIC1 and ASIC2a extracted from rat medulla (20 μg) were detected by Western-blotting, β-actin was served as a control. (c) Statistical data of (b) and the relative protein levels of ASIC1 and ASIC2a were decreased in adult v.s. neonatal samples. The protein levels of ASICs in neonatal rats were set as control. ***p < 0.001, n = 4.

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