Figure 2
The distribution of ASIC1 and ASIC2a in the medulla of neonatal and adult rats.
(a) and (b) ASIC1 positive cells in the VLM and DM of medulla in neonatal rats, respectively; (a1) and (b1 and b2) Representative high power visual field of area in (a) and (b), respectively. (c) Negative control. (d) and (e) ASIC1 positive cells in the VLM and DM of medulla in adult rats, respectively; (d1) and (e1 and e2) Representative high power visual field of area in (d) and (e), respectively. (f) Absorption control, and antibody was pre-absorbed by ASIC1. (g) Number of ASIC1 positive cells was decreased in adult rats compared to neonatal rats (***p < 0.001). (h) ROD of ASIC1-ir was increased in adult rats compared to neonatal rats (***p < 0.001). (i) ASIC2a positive cells in the VLM of medulla in neonatal rats; (i1) Representative high power visual field of area in (i). (j) No ASIC2a positive cell was found in the DM of medulla in neonatal rats. (k) Negative control; (l) and (m) ASIC2a positive cells in the VLM and DM of medulla in adult rats, respectively; (l1) and (m1 and m2) Representative high power visual field of area in (l) and (m), respectively. (n) Absorption control, and antibody was pre-absorbed by ASIC2a. (o) Number of ASIC2 positive cell in the VLM was decreased in adult rats compared to neonatal rats (***p < 0.001). (p) ROD of ASIC2-ir in VLM was also decreased in adult rats compared to neonatal rats (***p < 0.001). (q) Coronal diagram of the rat medulla, red area represented distribution of ASIC1 and ASIC2 (right) and the corresponding nuclei are marked out on the left half-side. ***p < 0.001, n = 6. Scale bar = 200 µM (a,b,c,d,e,f,i,j,k,l,m,n), = 25 µM (a1, b1, b2, d1, e1, e2, i1, l1, m1, m2). VLM: ventrolateral medulla; DM: dorsal medulla; RVL: rostroventrolateral reticular nucleus; LPGi: lateral paragigantocellular nucleus; In: intercalated nucleus of the medulla; MVe: medial vestibular nucleus; sol: solitary tract; Cu: cuneate nucleus; ECu: external cuneate nucleus.
