Figure 2

High vimentin expression correlated with TGF-β-induced EMT in oral squamous cell carcinoma cells.
(A) Immunofluorescence staining for vimentin in HN4 cells treated with or without TGF-β. (B) Western blot analysis of vimentin in HN4 cells treated with or without TGF-β. (C) RT-PCR analysis of vimentin mRNA levels in HN4 cells treated with or without TGF-β. (D) Representative images of Migration of HN4 cells treated with or without TGF-β in assay as described. (E) Bar graph showed the mean ± SD for the percent of migrated cells from 3 separate experiments, *P < 0.05.