Figure 5
RT-qPCR results for the three genes evaluated in the oral feeding bioassay (1 μg dsRNA/mL diet): prosα2, rps13 and snf7.
Prosα2 transcript levels were downregulated by 45% at day 2 after being placed on the dsRNA-containing diet, and by 73% at day 4 after being placed on the dsRNA-containing diet. For rps13, gene expression was downregulated by 28% at day 2 and by 66% at day 4after being placed on the dsRNA-containing diet. For snf7, this was 45% and 65%. The data was normalized to the expression levels in the control, which were fed dsRNA targeting the green fluorescent protein gene gfp. Statistical analysis was performed using the Student’s t-test in qBase+ and showed significant downregulation for all three genes at the different timepoints (*p < 0.05). Error bars represent the SEM.
