Figure 2 | Scientific Reports

Figure 2

From: The role of the unusual threonine string in the conversion of prion protein

Figure 2

PMCA propagation of recPrion.

Recombinant PrPa, PrPb, PrPL108F, or PrPT189V was used as the substrate for PMCA. Each PMCA reaction was carried out for 6 rounds and PMCA products were digested with 50 μg/mL PK at 37 °C for 30 min. PrP was detected by immunoblot analysis with POM1 anti-PrP antibody55. “C” indicates undigested recPrP as a control. The original immunoblot images are in the Supplementary Figure S3.

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