Table 2 Liquid chromatography mass spectrometry analyses of EcFrmR proteins treated with formaldehyde.
From: The mechanism of a formaldehyde-sensing transcriptional regulator
Protein sample | Measured mass (Da) | Relative abundance (a. u.) | Mass difference(Da)a | Commentb |
|---|---|---|---|---|
EcFrmR | 10186.60 | 2.0 × 107 | 0.1 | FrmR monomer |
20371.02 | 4.4 × 106 | −2.18 | FrmR disulfide-linked dimer | |
HCHO-treated EcFrmR | 10187.02 | 1.8 × 106 | 0.52 | FrmR monomer |
20396.87 | 2.0 × 105 | 23.67 | FrmR dimer with 2 methylene bridges | |
40768.73 | 2.0 × 105 | 22.30 | Two FrmR dimers, each with 1 methylene bridge, linked to form a tetramer by 1 disulfide bond | |
HCHO-treated EcFrmR plus Zn(II) | 10187.01 | 1.8 × 106 | 0.51 | FrmR monomer |
20396.95 | 3.0 × 105 | 23.75 | FrmR dimer with 2 methylene bridges | |
Zn(II)-treated EcFrmR plus HCHO | 10187.04 | 1.8 × 106 | 0.54 | FrmR monomer |
20396.99 | 4.0 × 105 | 23.79 | FrmR dimer with 2 methylene bridges | |
HCHO-treated EcFrmR(P2A) | 20319.69 | 4.0 × 105 | −1.11 | FrmR(P2A) disulfide-linked dimer |
HCHO-treated EcFrmR(P2A)c | 10190.71 | 6.8 × 105 | 29.91 | Hydroxymethylated FrmR(P2A) monomer |
HCHO-treated EcFrmR(C35A) | 20307.40 | 2.8 × 106 | −1.40 | FrmR(C35A) disulfide-linked dimer |
HCHO-treated EcFrmR(C35A)c | 20307.21 | 1.7 × 105 | −1.59 | FrmR(C35A) disulfide-linked dimer |
