Figure 2: Verification the expression of the secreted differentially expressed MAL-associated serum glycoproteins.

Western blotting detection was used to validate the differentially expressed MAL-associated glycoproteins (α-2-macroglobulin, α-1-antitrypsin, Gal-3BP and vitamin D-binding protein) in other pooled serum samples from 15 Chinese HCC patients with chronic HBV infection and from 15 healthy controls. The cropped blots are displayed: all of the four glycoproteins were completely coincident with the iTRAQ quantification trend. Bar graph of α-2-macroglobulin, α-1-antitrypsin, Gal-3BP and vitamin D-binding protein based on quantitative analysis of immunoblotted bands performed by Quantity One v4.62. ***p < 0.001.