Figure 5: Gal-3BP showed a relative sensitivity in the diagnosis of HCC with chronic HBV infection.

(A) Gal-3BP was immunoprecipitated in pooled serum samples from 15 early HCC patients with chronic HBV infection and 15 healthy controls, respectively. Lectin blotting detection was performed, and the cropped blots showed that the glycosylation of serum Gal-3BP was highly stable between HCC patients and healthy controls. (B) Scatter plot graphs of Gal-3BP intensity by ELISA analysis (serum samples from 80 HCC patients with chronic HBV infection and from 80 healthy controls) were analyzed by Student’s t-test, ***p < 0.001. Increased serum Gal-3BP levels in HCC patients with chronic HBV infection were detected. (C) ROC curve of serum Gal-3BP level. (D) ROC curve of the panel constructed with serum AFP level and serum Gal-3BP level.