Figure 3

PCA-LDA scores plot of ATR-FTIR spectra with regards to LD1: controls vs treated (normal cytology and HPV –ve); (a) together with absorbance per wavenumber (b). The 2 groups were significantly different along LD1 (Mean/SD (a): 0.32/0.10 for Controls; 0.20/0.11 for Treated; p < 0.0001, 95% CI = −0.18 to −0.07). These results evidence that the difference in LD1 was due to the impact of treatment. Absorbance associated with Amide II was shown to have a significant positive rate of change for the treated group compared with controls, indicating higher bioavailability (b). No other significant changes were detected. ATR-FTIR: Attenuated total reflection Fourier-transform Infrared; CI: Confidence interval; LD1: Linear Discriminant 1; -ve: Negative; PCA-LDA: Principal Component Analysis coupled to Linear Discriminant Analysis; +ve: Positive; SD: Standard deviation.