Figure 13 | Scientific Reports

Figure 13

From: Shengmai Formula suppressed over-activated Ras/MAPK pathway in C. elegans by opening mitochondrial permeability transition pore via regulating cyclophilin D

Figure 13

The opening of PTP promoted ROS generation and inhibited the over-activated Ras/MAPK pathway.

(A) Quantified fluorescence intensity of H2DCF-DA (N = 20). (B) Quantified fluorescence intensity of MitoSox (N = 20). (C) Quantified fluorescence intensity of JC-1 (N = 20). C: control. BA, 8 μM Bongkrekic acid; CsA, 5 μM Cyclosporin A; DIDS, 500 μM 4,4′-Diisothiocyanatostilbene -2,2′-disulfonic acid disodium salt hydrate; CCCP, 10 μM Carbonyl cyanide 3- chlorophenylhydrazone, positive control. (D) The effect of PTP inhibitor CsA, BA and DIDS on the Muv phenotype of let-60 (gf) mutants (N = 80–100). HT115 containing empty L4440 was fed as a control. CsA completely reversed the effect of SM, but not BA and DIDS. Data is presented as the mean ± SD of at least three independent experiments. Bars with different letters indicated that there was a significant difference at a level of 0.05 among groups.

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