Figure 4 | Scientific Reports

Figure 4

From: Shengmai Formula suppressed over-activated Ras/MAPK pathway in C. elegans by opening mitochondrial permeability transition pore via regulating cyclophilin D

Figure 4

SM increased the level of ROS accumulation in let-60 (gf) mutants.

(A) Fluorescence image of worms, which were stained by 10 μM H2DCF-DA at 20 °C for 20 min (N = 20). Con and C: control. NAC, 2.5 mM N-Acety-L-Cysteine; PQ, 0.5 mM paraquat. Scale bar, 200 μm. (B) Quantified fluorescence intensity of H2DCF-DA of each group. SM significantly increased the ROS accumulation. Data is presented as the mean ± SD of at least three independent experiments. Bars with different letters indicated that there was a significant difference at a level of 0.05 among groups.

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