Figure 3
DISHEVELLED and PDZ-RGS3 are not in the EFNB3 reverse signaling pathway in VSMCs.
Experiments in this figure were repeated more than twice, and representative data are shown. (A) Effective mRNA knockdown of Disheveled and PDZ-RGS3 by siRNA. VSMCs from female WT mice were transfected with siRNAs of Disheveled and PDZ-RGS3 as described in Fig. 2. The mRNA expression of each gene was determined by RT-qPCR. The data are expressed as means ± SD of the ratios of the target gene signal versus the β-actin signal. The data were analyzed by Student’s t test. *p < 0.05. (B) Dishevelled and PDZ-RGS3 knockdown by siRNAs had no effect on WT VSMC contractility. Contractility of VSMCs from female WT mice were assessed in the presence of EFNB3 reverse signalling and DISHEVELLED/PDZ-RGS3 knockdown, as described in Fig. 2B. The data were analyzed with one-way ANOVA but no significant difference was observed.
