Table 2 Echocardiographic analysis of CO, carotid artery resistance and left ventricle mass of Efnb3 KO and WT mice.

Mouse Type	Age	Cardiac output (ml/min)	Left Carotid PI	Right Carotid PI	LV Mass (mg)
Echocardiographic parameters of male Efnb3 KO mice
KO male	15wk	14.65	0.75	0.72	103.7
KO male	15wk	21.46	0.87	0.68	125.8
KO male	15wk	24.79	0.73	0.78	154.1
KO male	15wk	17.23	0.74	0.70	91.7
KO male	15wk	14.93	0.78	0.78	89.7
KO male	14wk	18.2	0.73	0.72	133.6
KO mean		18.54	0.77	0.73	116.4
SD		3.939	0.0533	0.041	25.65
WT male	14wk	21.5	0.83	0.84	116.0
WT male	15wk	15.33	0.74	0.70	140.5
WT male	15wk	23.52	0.75	0.77	115.6
WT male	15wk	18.21	0.73	0.72	133.6
WT male	14wk	31.85	0.77	0.86	115.0
WT male	14wk	28.57	0.77	0.81	145.1
WT male	14wk	24.19	0.74	0.76	152.7
WT male	14wk	30.95	0.79	0.83	175.6
WT mean		24.265	0.77	0.78	126.4
SD		5.92	0.030	0.047	12.57
p value (t test)		0.0637	0.9075	0.08200	0.4967
Echocardiographic parameters of female Efnb3 KO mice
KO female	15wk	21.89	0.75	0.78	112.3
KO female	15wk	16.29	0.77	0.8	92.5
KO female	14wk	20.16	0.81	0.81	121.1
KO female	15wk	27.57	0.76	0.77	143.3
KO female	15wk	20.54	0.94	0.76	130.5
KO female	14wk	27.71	0.75	0.73	134.3
KO female	15wk	19.13	0.8	0.77	135.8
KO female	14wk	18.38	0.81	0.82	122
KO mean		21.45	0.79	0.78	123.9
SD		4.153	0.062	0.029	16.04
WT female	14wk	33.49	0.72	0.72	109
WT female	14wk	18.92	0.69	0.67	118.1
WT female	14wk	29.03	0.71	0.8	110.9
WT female	14wk	28.06	0.81	0.79	95.9
WT female	14wk	15.96	0.68	0.76	114.6
WT female	14wk	22.46	0.7	0.74	72.2
WT mean		24.65	0.71	0.74	93.4
SD		6.664	0.047	0.048	29.98
p value (t test)		0.289	0.0218	0.132	0.0397

BP-related echocardiographic parameters of individual Efnb3 KO and WT mice are reported. Means ± SD are shown at the end of each group, and p values (unpaired one-way Student’s t test) are indicated at the bottom of Tables 2. PI: Pourcelot index; LV: left ventricle.

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