Figure 6

Pro-metastatic effect of miR-150 was partially attributed to targeted silencing of FOXO4.

(a) Analysis of the migratory capacity of H460 cells transiently transfected with miR-150 inhibitor individually and co-transfected with the miR-150 inhibitor and siFOXO4 using the Transwell assay. (b) Analysis of the migratory capacity of A549 cells transiently transfected with miR-150 inhibitor individually and co-transfected with the miR-150 inhibitor and siFOXO4 using the Transwell assay. The mean number of migrated cells was counted from at least five randomly selected fields. Data are the mean ± SD of three independent experiments. **p < 0.01 versus the control. (c) Western blot analysis of the expression of FOXO4, NF-κB, snail and E-cadherin in H460 and A549 cells transiently transfected with miR-150 inhibitor individually and co-transfected with the miR-150 inhibitor and siFOXO4. Cropped blots are displayed, and densitometry analysis and statistical analysis of protein bands were shown on the right. Experiments were performed in triplicate, and data are expressed as the mean ± SD, *p < 0.05 or **p < 0.01 versus the control. Full-length blot can be seen in Supplementary Fig. S4. Supplementary Fig. S4c corresponds to (c).