Figure 3 | Scientific Reports

Figure 3

From: The Identification and Characterization of Two Novel Epitopes on the Nucleocapsid Protein of the Porcine Epidemic Diarrhea Virus

Figure 3

The reactivity between epitopes and MAbs or antisera.

The two fusion-expressed epitopes (GST-NEP-D4 and GST-NEP-D6) and the negative control (GST tag) were processed and analyzed by Western blotting using PN-D4, PN-D6, or pig PEDV antisera obtained at 14 and 21 days after starting the immunization. Pig TGEV hyperimmune antisera was used as the primary antibodies in membranes a,b,c,d and e. (f) We used the pig TGEV hyperimmune antisera to test GST-TGEV-N protein and GST tag. In IFA, monolayers of Vero cells inoculated with the PEDV strain HLJ/2011 or FJzz1/2011 and ST cells inoculated with the TGEV strain SH/2012 were fixed for IFA against MAbs PN-D4, PN-D6 or anti-TGEV N protein. (g) The PEDV strain HLJ/2011 was detected by MAb PN-D4. (h) The PEDV strain HLJ/2011 was detected by MAb PN-D6. (j) The TGEV strain SH/2012 was detected by MAb PN-D4. (k) The TGEV strain SH/2012 was detected by MAb PN-D6. (i) The PEDV strain FJzz1/2011 was detected by the MAb anti-TGEV N protein. (l) the TGEV strain SH/2012 was detected by the anti-TGEV N protein.

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