Table 1 Primers designed to amplify the N gene and its truncations.

From: The Identification and Characterization of Two Novel Epitopes on the Nucleocapsid Protein of the Porcine Epidemic Diarrhea Virus

Segments		Sequences (5′-3′)	Positions (Amino Acid)
N1-1	N1-1U	CGGGATCCATGGCTTCTGTCAGCTTTCAGG	1–150
N1-1	N1-1L	GCCCTCGAGCCTGCTACGTGAATTTGCACG	1–150
N1-2	N1-2U	CGGGATCCATGGCTTCTGTCAGCTTTCAGG	1–140
N1-2	N1-2L	GCCCTCGAGAGGTGTGTTAGGTTCAACAATC	1–140
N1-3	N1-3U	CGGGATCCATGGCTTCTGTCAGCTTTCAGG	1–139
N1-3	N1-3L	GCCCTCGAGTGTGTTAGGTTCAACAATCTC	1–139
N1-4	N1-4U	CGGGATCCATGGCTTCTGTCAGCTTTCAGG	1–136
N1-4	N1-4L	GCCCTCGAGTTCAACAATCTCAACTACGCT	1–136
N1-5	N1-5U	CGGGATCCATGGCTTCTGTCAGCTTTCAGG	1–133
N1-5	N1-5L	GCCCTCGAGCTCAACTACGCTGGGAAGC	1–133
N1-6	N1-6U	CGGGATCCGTGCCATTATCCCTCTAT	1–130
N1-6	N1-6L	GCCCTCGAGGCTGGGAAGCTGTTGAGAGAAA	1–130
N1-7	N1-7U	CGGGATCCGTGCCATTATCCCTCTAT	14–133
N1-7	N1-7L	GCCCTCGAGCTCAACTACGCTGGGAAGC	14–133
N1-8	N1-8U	CGGGATCCGTGCCATTATCCCTCTAT	14–132
N1-8	N1-8L	GCCCTCGAGAACTACGCTGGGAAGCTGT	14–132
N1-9	N1-9U	CGGGATCCTCCCTCTATGCCCCTCTT	17–133
N1-9	N1-9L	GCCCTCGAGCTCAACTACGCTGGGAAGC	17–133
N1-10	N1-10U	CGGGATCCCTCTATGCCCCTCTTAGGGT	18–133
N1-10	N1-10L	GCCCTCGAGCTCAACTACGCTGGGAAGC	18–133
N1-11	N1-11U	CGGGATCCTATGCCCCTCTTAGGGTTAC	19–133
N1-11	N1-11L	GCCCTCGAGCTCAACTACACTGGGGAGC	19–133
N2-1	N2-1U	CGGGATCCAAGCTTAAGCAACAGCAGA	241–442
N2-1	N2-1L	GCCCTCGAGTTAATTTCCTGTATCGAAGAT	241–442
N2-2	N2-2U	CGGGATCCAAGCTTAAGCAACAGCAGA	241–341
N2-2	N2-2L	GCCCTCGAG ACGAACAGCCACATTACC	241–341
N2-3	N2-3U	CGGGATCCAAGCTTAAGCAACAGCAGA	241–308
N2-3	N2-3L	GCCCTCGAGTTCCGCATCTCCAAAAT	241–308
N2-4	N2-4U	GATCCAAGCTTAAGCAACAGCAGAAGCCCAAACAGGAAAGGTCTGACAGCAGCGGCAAAAATACACCTAAGAAGC	241–263
N2-4	N2-4L	TCGAGCTTCTTAGGTGTATTTTTGCCGCTGCTGTCAGACCTTTCCTGTTTGGGCTTCTGCTGTTGCTTAAGCTTG	241–263
N2-5	N2-5U	GATCCCAACAGCAGAAGCCCAAACAGGAAAGGTCTGACAGCAGCGGCAAAAATACACCTAAGAAGC	244–263
N2-5	N2-5L	TCGAGCTTCTTAGGTGTATTTTTGCCGCTGCTGTCAGACCTTTCCTGTTTGGGCTTCTGCTGTTGG	244–263
N2-6	N2-6U	GATCCCAGGAAAGGTCTGACAGCAGCGGCAAAAATACACCTAAGAAGC	250–263
N2-6	N2-6L	TCGAGCTTCTTAGGTGTATTTTTGCCGCTGCTGTCAGACCTTTCCTGG	250–263
N2-7	N2-7U	GATCC CAGGAAAGGTCTGACAGCAGCGGCAAAAATACACCTAAGC	250–262
N2-7	N2-7L	TCGAG CTTAGGTGTATTTTTGCCGCTGCTGTCAGACCTTTCCTGG	250–262
N2-8	N2-8U	GATCCCAGGAAAGGTCTGACAGCAGCGGCAAAAATACACCTC	250–261
N2-8	N2-8L	TCGAGAGGTGTATTTTTGCCGCTGCTGTCAGACCTTTCCTGG	250–261
N2-9	N2-9U	GATCC GAAAGGTCTGACAGCAGCGGCAAAAATACACCTAAGC	251–262
N2-9	N2-9L	TCGAG CTTAGGTGTATTTTTGCCGCTGCTGTCAGACCTTTCG	251–262
N2-10	N2-10U	GATCC AGGTCTGACAGCAGCGGCAAAAATACACCTAAGC	252–262
N2-10	N2-10L	TCGAG CTTAGGTGTATTTTTGCCGCTGCTGTCAGACCTG	252–262
N2-11	N2-11U	GATCC TCTGACAGCAGCGGCAAAAATACACCTAAGC	253–262
N2-11	N2-11L	TCGAG CTTAGGTGTATTTTTGCCGCTGCTGTCAGAG	253–262

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Table 1 Primers designed to amplify the N gene and its truncations.

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