Figure 2: Effect of CHA on macrophage marker expression induced by interleukin (IL)-4. | Scientific Reports

Figure 2: Effect of CHA on macrophage marker expression induced by interleukin (IL)-4.

From: Chlorogenic acid inhibits glioblastoma growth through repolarizating macrophage from M2 to M1 phenotype

Figure 2

Ana-1 and RAW264.7 cells were treated with interleukin (IL)-4 (20 ng/ml) in the presence of DMSO or different concentrations of CHA for 24 h. The mRNA levels of M1-marker gene iNOS and M2-marker gene Arg in Ana-1 (A) and RAW264.7 cells (B) were measured by real-time RT-PCR. The expression of mRNAs was normalized to GAPDH. The expressions of CD206 in Ana-1 (C) and RAW264.7 cells (D) were evaluated by flow cytometry. The histogram bars represent three independent experiments. The data are presented as the mean ± SD. *p-value < 0.05, **p-value < 0.01 vs.IL-4.

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