Figure 3
Effect of IGF1 rs5742714 polymorphism on IGF1 expression.
(A) Association between IGF1 expression in renal tissues and IGF1 rs5742714 genotypes. Compared to individuals with the rs5742714 GG genotype, individuals with the rs5742714 GC or CC genotype were associated with significantly decreased IGF1 mRNA levels (P = 0.013 and P = 0.017, respectively); (B) Bioinformatics analysis predicted a binding site between miR-580 and IGF1; (C) Transient transfection of reporter and mimics into HEK293 and 786-o cell lines. Luciferase activity was measured using a Dual-Luciferase Reporter Assay System (Promega). Values are mean ± SD from more than three separate experiments that were each performed in triplicate. *Indicates a significant difference (P < 0.05). There is significant difference between Group C, which were transfected with miR-580 mimics and pGL3-IGF1-G and Group D, which were transfected with miR-580 mimics and pGL3-IGF1-C.
