Figure 5
IL-8 treatment might not alter the interaction properties between REEP5, REEP6, and CXCR1.
(A) HEK293 cells expressing CXCR1-GFP and His-tagged REEP6 were treated with IL-8, fixed, and prepared for immunocytochemistry using anti-His antibodies and secondary Alexa 594-conjugated antibodies. Fluorescence signals were observed by confocal microscopy. The scale bar denotes 10 μm. (B) HEK293 cells expressing FLAG-REEP6 and HA-CXCR1 were treated with IL-8 for the indicated times and subjected to immunoprecipitation with anti-HA antibodies. Co-precipitated REEP6 was detected by western blotting with anti-FLAG antibodies. The results are from at least three independent experiments.
