Figure 7
Depletion of REEP5 and REEP6 weakens the proliferation and invasion abilities of A549 cells.
A549 cells containing shRNAs were seeded in 96-well plates (2,000/well), and cultured with (A) medium containing 10% FBS or (B) serum-free medium containing 100 ng/ml of IL-8. At 24 h, 48 h, 72 h, and 96 h after treatment, the cells were incubated with a CCK-8 solution for 2 h, and a microplate reader was used to measure absorbance at 450 nm. (C) A549 cells (5 × 104) were seeded in the upper chamber of a Transwell® plate and treated with 100 ng/ml of IL-8. Medium containing 10% FBS was added to the lower well. Eighteen hours later, the Transwell® inserts were removed and stained with hematoxylin. (D) The cells that migrated were counted under 200x magnification in five microscope fields. The average number of cells that migrated per field is shown in the graph. *p < 0.05; **p < 0.01. The results are from at least three independent experiments.
