Figure 8
The tumorigenesis and lung metastasis of A549 cells is suppressed by the silencing of REEP5 and REEP6.
(A) A549 cells containing shRNAs were injected into the flank of NOD/SCID mice. Tumor volumes were measured every week and calculated using the following formula: 0.5 × length × width2 (mm3) (n = 6 mice per group, n = 5 of the REEP5 and REEP6-shRNA groups due to accidental death during the experiment). **Statistical significance of the difference between the REEP5 and REEP5/6 shRNA groups from controls; p < 0.01. (B) Ten weeks after implantation, the tumors were resected for analysis. The pictures show the tumors from each group. The volume (C) and weight (D) of each tumor were measured and presented graphically. The results are expressed as mean ± S.E.; *p < 0.05. (E) The mice were sacrificed 14 weeks after injection and lung sections were examined by hematoxylin/eosin staining. Pictures are representative of lung section data. Scale bar, 200 μm. (F) The tumor nodules in lungs of mice were counted. (G) The metastatic ability was represented as tumor burden. Results are expressed as mean ± S.E.; *p < 0.05; **p < 0.01.
