Figure 4 | Scientific Reports

Figure 4

From: Mitochondrial-bacterial hybrids of BamA/Tob55 suggest variable requirements for the membrane integration of β-barrel proteins

Figure 4

Cells harboring hybrid Tob55/BamA proteins contain lower levels of PhoE.

(a) Crude mitochondria were isolated from Tob55-depleted cells co-expressing OmpA and either plasmid-encoded Tob55 or the indicated hybrid protein. Proteins were analyzed by SDS-PAGE and immunodetection with antibodies against OmpA, and the indicated mitochondrial proteins. (b) Crude mitochondria were isolated from Tob55-depleted cells co-expressing PhoE and the indicated hybrid protein. Further treatment and analysis were as described in part (a). (c) The bands of at least three experiments as those described in (a) and (b) were quantified and their means ± s.d. are presented. To allow comparison among the various samples, the levels of Tom20 were taken as internal reference. The intensities of the bands from cells transformed with native Tob55 (construct A) were set to 100%. **p value < 0.01. A schematic representation of the relevant Tob55 variants is included.

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