Table 1 Properties of Tob55/BamA hybrids.

From: Mitochondrial-bacterial hybrids of BamA/Tob55 suggest variable requirements for the membrane integration of β-barrel proteins

Variant

Replacement of Tob55 or BamA

Membrane insertion

Assembly into TOB complex

Yeast

E. coli

Yeast

E. coli

A (Tob55)

yes

no

yes

part.

yes

B

part.

no

yes

part.

nd

C

no

no

part.

part.

nd

D

yes

no

yes

yes

yes

E

no

no

part.

part.

nd

F (BamA)

no

yes

part.

part.

no

G

yes

nt

yes

nt

yes

H

no

nt

part.

nt

no

J

nt

no

nt

part.

nt

  1. nt, construct was not tested in this experiment; part., partially; nd, hybrid protein was not detectable.