Figure 2

SRGAP2 mRNA xpression levels in K12 and K7M2 cell lines by quantitative RT-PCR and protein quantification by Western blotting.

(a,b) SRGAP2 mRNA levels were quantified by RT-PCR. The expression of SRGAP2 mRNA is >2 fold overexpressed in cell lines with the addition of doxycycline. Expression of Srgap2 mRNA is (a) halved in the K12 KO and (b) not detectable in the K7M2 KO. Bar graph shows mean ± SEM (n = 3). (c,d) Western blotting showing the addition of doxycycline to culture media induces expression of human SRGAP2 protein in the (c) K12 and (d) K7M2 OE cell lines. The antibody does not bind endogenous murine SRGAP2 protein. Four bands are observed: SRGAP2 (140 KDa), beta-actin control (42 KDa), and a two unknown bands (120 KDa and 100 KDa). ****p-value < 0.0001 (two-tailed, unpaired t-test).