Figure 3
From: Multivalent pIX phage display selects for distinct and improved antibody properties
Effect of scFv capsid fusion expression and translocation mechanism on E. coli growth.
(a,b) Two random, independent clones each representing full-length scFv, or scFv containing in-frame truncations, or stop codons displayed on either (a) pIII or (b) pIX were grown in the presence of glucose repressor to shut down scFv expression, or during LV and HV phage packaging as indicated. (c) A model scFv clone was displayed on pIII with pelB or on pIX with or without pelB, and E. coli growth was monitored in the presence of glucose, or during LV and HV phage packaging as indicated. (*) super infection, (**) medium exchange. Results are representative of three independent experiments.
