Figure 1
Naa50/San is required for the correct interaction between Scc1 and Smc3.
(A) Wing blade-specific RNAi using the Nubbin-Gal4 driver43,44 and the UAS/Gal4 system42. (B) Adult wings of wild type Drosophila (Oregon R; OR), Drosophila expressing a control RNAi (mCherry RNAi), and Drosophila expressing RNAi for san in the larvae wing imaginal discs. (C) Scored adult wing phenotypic classes: class 1 (wild type wings); class 2 (weak wing phenotype); class 3 (san RNAi-like wing phenotype); class 4 (highly abnormal wings); class 5 (absence/vestigial adult wings). (D) Quantification of Drosophila wing phenotypes expressing individual RNAi transgenes for mCherry, deco, scc1 or mau-2 (grey bars) or co-expressing san RNAi with mCherry RNAi, deco RNAi, scc1 RNAi, or mau-2 RNAi (black bars) in the larvae wing imaginal discs. (E) Representative adult wing phenotype of Drosophila co-expressing san RNAi with mCherry RNAi, deco RNAi, scc1 RNAi or mau-2 RNAi in the larvae wing imaginal discs. (F) Quantification of Drosophila wing phenotypes expressing a RNAi transgene for mCherry, a Smc3-Scc1 fusion construct58 or overexpressing Dmt in the larva wing imaginal discs, and Drosophila without one copy of Dmt (dmt+/−) (grey bars). Quantification of Drosophila wing phenotypes co-expressing san RNAi with mCherry, a Smc3-Scc1 fusion construct or Dmt in the larvae wing imaginal discs, and Drosophila without one copy of Dmt (dmt+/−) expressing san RNAi in the larvae wing imaginal discs (black bars). (G) Representative adult wing phenotype of Drosophila co-expressing san RNAi with mCherry RNAi, a Smc3-Scc1 fusion construct or Dmt in the larvae wing imaginal discs, and Drosophila without one copy of Dmt (dmt+/−) expressing san RNAi in the larvae wing imaginal discs. Phenotypic quantification of adult wings is mean ± S.D. of three independent experiments and is based on the classes described in (C) and Supplementary Fig. 1 (**p < 0.01 and ***p < 0.001, Student’s t test; n represents the total number of flies evaluated). The genotypes are written in green, black, or red if there was, respectively, suppression, no alteration, or enhancement of the original san RNAi wing phenotype. Detailed genotypes are indicated in Supplementary Table 1. Top left panels in (E) and (G), and wild-type panel in (B) and Class1 panel in (C), are the same.
