Figure 2
BRU down-regulates HIF-1α protein levels in hypoxic conditions.
(A) HCT116 cells were treated with hypoxia (1% O2) for different time and the quantity of HIF-1α protein was analyzed by Western blot. (B) The relative quantity of HIF-1α protein described in (A). (C) HCT116 cells were treated with 200 μM CoCl2-induced mimic hypoxia for different time and the quantity of HIF-1α protein was analyzed by Western blot. (D) The relative quantity of HIF-1α protein described in (C). (E) HCT116 cells were treated with various concentrations of BRU for 4 h under hypoxia (1% O2) and the quantity of HIF-1α protein was analyzed by Western blot. (F) The relative quantity of HIF-1α protein described in (E). Data was presented as means ± S.D. (n = 3). ***p < 0.001 and *p < 0.05 versus the hypoxia alone group. (G) HCT116 cells were treated with various concentrations of BRU for 4 h under 200 μM CoCl2-induced mimic hypoxia and the quantity of HIF-1α protein was analyzed by Western blot. (H) The relative quantity of HIF-1α protein described in (G). Data was presented as means ± S.D. (n = 3). ***p < 0.001 and **p < 0.01 versus the mimic hypoxia alone group.
