Figure 5
BRU inhibits the transactivation function of HIF-1 in hypoxic conditions.
HCT116 cells were transiently cotransfected with the HRE-luciferase plasmid and an internal control vector pRL-TK for 24 h and then treated with or without BRU for 6 h under hypoxia (A) or under CoCl2-induced mimic hypoxia (B), and then luciferase activity was quantitated. Data was presented as means ± S.D. (n = 6). ***p < 0.001, **p < 0.01 and *p < 0.05 versus the hypoxia alone group in (A). ***p < 0.001 versus the mimic hypoxia alone group in (B).
