Figure 6
BRU down-regulates the expression of VEGF in HCT116 cells in hypoxic conditions.
(A) HCT116 cells were treated with various concentrations of BRU for 12 h under normoxia or hypoxia (1% O2). The mRNA levels of VEGF were analyzed by qRT-PCR and normalized with β-actin mRNA levels. Data was presented as means ± S.D. (n = 3). ***p < 0.001 versus the hypoxia alone group. (B) HCT116 cells were treated with various concentrations of BRU for 12 h under 200 μM CoCl2-induced mimic hypoxia. The mRNA levels of VEGF were analyzed by qRT-PCR and normalized with β-actin mRNA levels. Data was presented as means ± S.D. (n = 3). **p < 0.01 and *p < 0.05 versus the mimic hypoxia alone group. (C) HCT116 cells were treated with various concerntrations of BRU for 24 h under hypoxia and the quantity of VEGF protein was analyzed by Western blot. (D) HCT116 cells were treated with various concerntrations of BRU for 24 h under 200 μM CoCl2-induced mimic hypoxia and the quantity of VEGF protein was analyzed by Western blot. (E) The relative quantity of VEGF protein described in (C). Data was presented as means ± S.D. (n = 3). ***p < 0.001 versus the hypoxia alone group. (F) The relative quantity of VEGF protein described in (D). Data was presented as means ± S.D. (n = 3). *p < 0.05 versus the mimic hypoxia alone group.
