Figure 7
BRU inhibits expression of glycolytic enzymes and glucose consumption in HCT116 cells under hypoxia.
(A) HCT116 cells were treated with various concentrations of BRU for 12 h under hypoxia (1% O2). (B), (C) and (D) The mRNA levels of GLUT1, HK2 and LDHA were analyzed by reverse transcription-PCR and normalized with β-actin mRNA levels. Data was presented as means ± S.D. (n = 3). ***p < 0.001 versus the hypoxia alone group (0 nM BRU-treated group). (E) HCT116 cells were treated with various concentrations of BRU for 24 h under hypoxia (1% O2) and then glucose consumption was measured with commercial kit. Data was presented as means ± S.D. (n = 4). ***p < 0.001 versus the hypoxia alone group (0 nM BRU-treated group).
