Figure 8
BRU decreases intracellular ROS and mitochondrial ROS levels in HCT116 cells.
(A) HCT116 cells were treated with 60 nM BRU for 3 h and intracellular ROS generation was analyzed by DCFH-DA using confocal microscope (magnification, 200×). (B) HCT116 cells were treated with various concentrations of BRU for 3 h and intracellular ROS generation was analyzed by DCFH-DA using flow cytometry. (C) The fluorescence intensity of DCF described in (B) was quantitated. Data was presented as means ± S.D. (n = 3). ***p < 0.001 and **p < 0.01 versus the control group. (D) HCT116 cells were treated with 60 nM BRU for 3 h under hypoxia (1% O2) and intracellular ROS generation was analyzed by DCFH-DA using flow cytometry. (E) The fluorescence intensity of DCF described in (C) was quantitated. Data was presented as means ± S.D. (n = 3). ***p < 0.001 versus the normoxia group, ##p < 0.01 versus the hypoxia group. (F) HCT116 cells were treated with 60 nM BRU for 3 h under hypoxia (1% O2) and mitochondrial ROS generation was analyzed by mitoSOX Red using flow cytometry. (G) The fluorescence intensity of mitoSOX described in (F) was quantitated. Data was presented as means ± S.D. (n = 3). ***p < 0.001 versus the normoxia group, #p < 0.05 versus the hypoxia group.
