Figure 5 | Scientific Reports

Figure 5

From: Drosophila microRNA-34 Impairs Axon Pruning of Mushroom Body γ Neurons by Downregulating the Expression of Ecdysone Receptor

Figure 5

Overexpression of EcR-B1 rescued the miR-34-induced defective γ lobe phenotype in MB neurons.

Confocal images show the lobe pruning phenotypes of MB neurons in flies with ectopic overexpression of EcR-B1 (a), ectopic overexpression of miR-34 (b), or the overexpression of both (c). FasII staining reveals the medial γ lobes (arrowheads, faint magenta staining) and dorsal α and medial β lobes (arrows, strong magenta staining). In the lower panels, GAL4-OK107-driven mCD8::GFP (GFP) expression (green) shows the morphology of α lobes (arrows), α′ lobes (double arrows), and γ lobes (arrowheads). Overexpression of EcR-B1 in MB neurons had no observable effect on the MB lobe pruning phenotype (a). Ectopic miR-34 overexpression in MB neurons caused defective γ lobe pruning (double-arrowheads, b). The defective γ lobe pruning phenotype was rescued by overexpression of EcR-B1 in MB neurons with ectopic miR-34 overexpression (c). Fly genotypes are listed in Supplemental Table 2. Scale bar: 10 μm for all panels.

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