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Figure 1

From: Rats overexpressing the dopamine transporter display behavioral and neurobiological abnormalities with relevance to repetitive disorders

Figure 1

Generation of DAT-tg rats.

(a) Schematic representation of the 4-kb DNA fragment used for the generation of the DAT transgenic rats. E1/2 = exon 1/2, and I1 = intron 1 of NSE, mDAT = murine DAT sequence, SV40 = Simian virus 40. (b) Representative DAT PCR products from wt (−) and DAT-tg (+) rats. M = marker, NC = negative control, PC = positive control, transgenic band = 356 bp. One founder line was used for the study. Here one litter from this generation is shown. (c) Representative coronal sections of immunohistochemical stain of DAT expression for wt (left) and DAT-tg (right). (d) DAT Western blot analysis of striatal tissue from wt (n = 5) and DAT-tg rats (n = 10).

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