Figure 1
From: Phospholipase D1 deficiency in mice causes nonalcoholic fatty liver disease via an autophagy defect
Pld1 expression is decreased in HFD-induced hepatic steatosis.
(a) C57BL/6 mice were fed either RC or a HFD for 4 weeks (n = 10 per group) starting at the age of 13 weeks and fasted overnight prior to collection of the liver. mRNA was isolated from the liver for qRT-PCR. (b) Mice were maintained as in (a) and the lysates were analyzed by western blot. Western blot results were analyzed by densitometry to obtain the relative ratio of either PLD1 or PLD2 to tubulin. The data are presented as means ± SE or representative blots from 3 to 5 independent experiments. *P < 0.05 versus the RC group. Abbreviations: HFD, high-fat diet; PLD2, phospholipase D2; RC, regular chow; SE, standard error; qRT-PCR quantitative real-time polymerase chain reaction.
