Figure 1
Immunogold labeling TEM within Lewy bodies from PD brains.
(a) Shows a Lewy body taken from PD041 case (Table 1) and (b,c) show representative micrographs showing double labeling using the dityrosine antibody (15 nm) and anti-α-syn (5 nm) antibody on a PD substantia nigra brain section taken from PD028 case (Table 1) and independent areas at increasing magnifications. The micrographs reveal very dense anti-α-syn labeling of the Lewy bodies and confirm colocalization of dityrosine with α-syn within Lewy bodies. (b) (ii) reveals fibrillar structures labeled with both 5 and 15 nm gold particles supporting the view that dityrosine crosslinks are present within the fibrils in the Lewy bodies (white arrows). (c) (i, ii and iii) highlight these structures by increasing magnification to show the presence of both 5 and 15 nm gold labels and the 15 nm (red) and 5 nm (blue) have been highlighted in (c) (iii). An area outside of the Lewy body is highlighted (dotted circle) to show the absence of gold labeling.
